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Transposon-mediated insertion of R factor into bacterial chromosome

โœ Scribed by Danilevich, V. N. ;Stepanshin, Y. G. ;Volozhantsev, N. V. ;Golub, E. I.

Book ID
104725454
Publisher
Springer
Year
1978
Tongue
English
Weight
182 KB
Volume
161
Category
Article
ISSN
0026-8925

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โœฆ Synopsis

Insertion of transposon T n1 into the E. coli JC411 chromosome results in a sharp increase of plasmid RP4 integration frequency. This effect is absent in JC1553 recA cells. The RP4 integration with the chromosome is probably accomplished via recA-dependent recombination between transposon Tn1 inserted into the chromosome and the same transposon in the RP4 plasmid.

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Tn10 mediated integration of the plasmid
Tn10 mediated integration of the plasmid R100.1 into the bacterial chromosome: Inverse transposition
โœ Chandler, M. ;Roulet, E. ;Silver, L. ;de la Tour, E. Boy ;Caro, L. ๐Ÿ“‚ Article ๐Ÿ“… 1979 ๐Ÿ› Springer ๐ŸŒ English โš– 879 KB

Upon integration into the bacterial chromosome the drug resistance plasmid R100.1 often loses its tetracycline resistance character. We have analyzed an Hfr strain formed by such an integration and an R-prime plasmid derived from it. We find that integration took place within the Tn10 transposon, th