Transport und Umsatz von Polyalkoholen bei der HefeRhodotorula gracilis (glutinis)

✍ Scribed by Renate Klöppel; Milan Höfer

• Publisher: Springer
• Year: 1976
• Tongue: English
• Weight: 583 KB
• Volume: 107
• Category: Article
• ISSN: 0302-8933
• DOI: 10.1007/bf00425348

✦ Synopsis

The obligate aerobic yeast Rhodoturula gracilis was found to take up the alditols D-glucitol, D-mannitol, ribitol, xylitol, D-arabinitol, L-arabinitol and erythritol by means of a constitutive mobile membrane carrier. This uptake involved active transport, that is, it was dependent on the supply of metabolic energy, leading to the accumulation of alditols inside the cells. The accumulation ratio (intracellular concentration to extracellular concentration, Si/SO) was much lower for alditols than for monosaccharides. As for monosaccharides, this ratio decreased with increasing extracellular concentration, even to values below 1. The kinetic data showed that the carrier system for alditols was identical to that for monosaccharides, though it had a much lower affinity and maximum velocity for alditols. Hence the uptake of alditols was blocked in the presence of monosaccharides. Only ribitol and L-arabinitol were catabolized following enzyme induction. The other alditols were not broken down.