Transport systems of isolated hepatocytes
β Scribed by Michael Schwenk
- Publisher
- Springer-Verlag
- Year
- 1980
- Tongue
- English
- Weight
- 788 KB
- Volume
- 44
- Category
- Article
- ISSN
- 0340-5761
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π SIMILAR VOLUMES
Cells were isolated from the liver of the skate and the uptake of beta-alanine followed using [14C]-beta-alanine. The isolated hepatocytes showed good viability, were found to accumulate beta-alanine from the incubation medium, and did so in a manner indicating a transport system involving a saturab
The effect of amino acid depletion or supplementation and the effect of glucagon and insulin on the amino acid transport mediated by s stem A were acid (AIB) or N-methyl 2-amino [l-'4C]isobutyric acid (MeAIB) in rat hepatocytes, freshly isolated at different stages of pre-and postnatal development.
The present report shows that System A-mediated 2-aminoisobutyric acid (AIB) uptake is elevated in hepatocytes isolated from adrenalectomized rats when they are compared to control cells. Although System ASC activity also shows this perturbation, Systems N, p, L1, and L2 are unaffected. Transport of