Transport-Specific isolation of large channels reconstituted into lipid vesicles

Isolation of the alanine carrier from th

Isolation of the alanine carrier from the membranes of a thermophilic bacterium and its reconstitution into vesicles capable of transport

✍ Hirata, Hajime ;Sone, Nobuhito ;Yoshida, Masasuke ;Kagawa, Yasuo 📂 Article 📅 1977 🏛 Wiley (John Wiley & Sons) 🌐 English ⚖ 425 KB

## Abstract A carrier protein mediatine alanine transport was purified from the membranes of the thermophilic bacterium PS3, by ion exchange chromatography in the presence of both Triton X‐100 and urea. The alanine carrier was recovered in the nonadsorbed fraction from either DEAE‐or CM‐cellulose