We have constructed three plasmid vectors for the expression of green fluorescent protein (GFP) fusion proteins using the following motif: (His) 6 -GFP-EK-X, where X represents chloramphenicol acetyl-transferase (CAT), human interleukin-2 (hIL-2), and organophosphorous hydrolase (OPH), respectively,
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Transport proteins PotD and Crr of Escherichia coli, novel fusion partners for heterologous protein expression
โ Scribed by Kyung-Yeon Han; Hyuk-Seong Seo; Jong-Am Song; Keum-Young Ahn; Jin-Seung Park; Jeewon Lee
- Publisher
- Elsevier Science
- Year
- 2007
- Tongue
- English
- Weight
- 999 KB
- Volume
- 1774
- Category
- Article
- ISSN
- 1570-9639
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Covalently immobilized biotin was used as a biospecific adsorbant to investigate the application of streptavidin as an affinity domain for simultaneous purification and immobilization of recombinant proteins. A streptavidinp-galactosidase fusion protein was constructed and tested as a model system.