Transport of leucine in the cyanobacteriumAnabaena variabilis

✍ Scribed by Teresa Thiel

• Publisher: Springer
• Year: 1988
• Tongue: English
• Weight: 501 KB
• Volume: 149
• Category: Article
• ISSN: 0302-8933
• DOI: 10.1007/bf00425589

✦ Synopsis

The amino acid leucine was transported by the cyanobacterium Anabaena variabilis. The Km for transport was 10.8gM; the Vm,x was 8.7nmolesmin-~mg -~ chlorophyll a. Transport of leucine was energy dependent: uptake of leucine was inhibited in the dark, and by DCMU and cyanide. Transport was neither dependent on nor enhanced by Na +. Prior growth of cells with leucine did not repress transport of [a4C]-leucine. Alanine, glycine, valine, and methionine were strong competitive inhibitors of leucine uptake; serine, threonine, isoleucine, norleucine, and D-alanine competitively inhibited to a lesser degree. Other amino acids or amino acid analogues, including D-leucine, c~-aminoisobutyrate, and D-serine did not inhibit the transport of leucine.