As a first approach towards studying the regulation of hemolysin synthesis in Escherichia coli, we have fused lacZ into the four hly genes (hlyC, hlyA, hlyBa and hlyBb) using the Mud-1 (Mu::lacZ, Y, Apr) phage. The sites of insertion of Mud-1 within the various hly genes of the Hly plasmid pHly152 w
Transport of hemolysin by Escherichia coli
✍ Scribed by Michael Härtlein; Sigrid Schießl; Wilma Wagner; Ursula Rdest; Jürgen Kreft; Werner Goebel
- Publisher
- John Wiley and Sons
- Year
- 1983
- Tongue
- English
- Weight
- 966 KB
- Volume
- 22
- Category
- Article
- ISSN
- 0730-2312
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A sequence (hlyR) of about 600 bp which enhances the expression of hemolysin (HlyA) more than 50-fold was identified in the plasmid pHly152-specific hemolysin (hly) determinant. Deletion of this entire hlyR sequence led to the same low level of hemolysin synthesis and excretion as that expressed by
IS91 is a 1.85 kb insertion sequence originally resident in the ~-hemolytic plasmid pSU233. The element was transposed sequentially from this plasmid to pA-CYC184, to R388, and to pBR322. Both cointegrates and simple insertions of the element were obtained. A detailed restriction enzyme map of the e
## Abstract Leucine is transported into E coli by two osmotic shock‐sensitive, high‐affinity systems (LIV‐I and leucine‐specific systems) and one membrane bound, low‐affinity system (LIV‐II). Expression of the high‐affinity transport systems is altered by mutations in __liv R__ and __1st R__, genes