TRANSPORT OF GLUTATHIONE S-CONJUGATES IN THE YEASTSSACCHAROMYCES CEREVISIAE

✍ Scribed by ROLAND ŻĄDZIŃSKI; JANUSZ MASZEWSKI; GRZEGORZ BARTOSZ

• Book ID: 102564898
• Publisher: Elsevier Science
• Year: 1996
• Tongue: English
• Weight: 226 KB
• Volume: 20
• Category: Article
• ISSN: 1065-6995
• DOI: 10.1006/cbir.1996.0035

✦ Synopsis

Abstract

Transport of 2,4‐dinitrophenyl‐S‐glutathione (DNP‐SG) and a fluorescent glutathione S‐conjugate, bimane‐S‐glutathione (B‐SG) was studied in the baker's yeasts (S. cerevisiae). Both conjugates were exported from the cells; the transport was inhibited by fluoride and vanadate like in mammalian cells. B‐SG was also found to be accumulated in the vacuoles. The transport rate of DNP‐SG outside the cell was higher in a vacuolar‐deficient strain. A significant ATP‐dependent uptake of (^3^H)‐DNP‐SG by vacuoles was found. These results indicate that__S. cerevisiae__transport glutathione S‐conjugates both outside the cells and into the vacuoles.