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Transport of carnitine in RBE4 cells - an in vitro model of blood-brain barrier

✍ Scribed by Joanna E. Mroczkowska; Françoise S. Roux; Hans-Joachim Galla; Maciej J. Nalecz; Katarzyna A. Nalecz

Publisher
John Wiley and Sons
Year
1996
Tongue
English
Weight
514 KB
Volume
19
Category
Article
ISSN
0893-6609

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✦ Synopsis

Accumulation of carnitine was studied in immortalized rat brain capillary endothelial cells R B E ~, as an in vitro model of blood-brain barrier. Both uptake and efflux phenomena were found independent on "a+] gradient. A quick acylation of carnitine in RBE4 cells was observed, especially leading to formation of long-chain acylcarnitines (46%), while 38% of the accumulated compound was found in the form of free carnitine. Exposure of basolateral membrane to carnitine-free medium induced a quick efflux, leaving, however, about 50% of accumulated carnitine inside the Cells. The Process of accumulation was found to be sensitive to butyrobetaine and cysteine, but not to choline or Hemicholinium-3, pointing to sterical demands of a transporter responsible for carnitine uptake.

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