Transplantation of microcarrier-attached hepatocytes into 90% partially hepatectomized rats

✍ Scribed by Achilles A. Demetriou; Andrew Reisner; Jay Sanchez; Stanley M. Levenson; Albert D. Moscioni; J. Roy Chowdhury

• Book ID: 102236775
• Publisher: John Wiley and Sons
• Year: 1988
• Tongue: English
• Weight: 496 KB
• Volume: 8
• Category: Article
• ISSN: 0270-9139
• DOI: 10.1002/hep.1840080505

✦ Synopsis

We previously reported a method of intraperitoneal transplantation of liver cells attached to collagen-coated microcarriers, which resulted in prolonged survival and function of the transplanted cells. In the present study, we evaluated the efficacy of liver cell transplantation in providing metabolic support during acute liver insufficiency induced by 90% partial hepatectomy in rats. Ninety per cent of the liver mass (all lobes except the caudate lobe) was resected, and the rats were provided with 5% dextrose orally ad libitum upon regaining consciousness. This regimen results in severe hypoglycemia and death within 48 hr. When microcarrier-attached liver cells were transplanted into syngeneic and allogeneic recipients 3 days prior to 90% partial hepatectomy, significantly higher blood glucose levels were observed (p < 0.01), compared to the levels in control rats which