๐”– Bobbio Scriptorium
โœฆ   LIBER   โœฆ

Transmission electron microscopy procedure for endothelial cell cultures with angiogenesis

โœ Scribed by Laurace E. Townsend; John L. Glover; Marilyn L. Seymour

Publisher
Springer
Year
1996
Tongue
English
Weight
451 KB
Volume
18
Category
Article
ISSN
1573-0603

No coin nor oath required. For personal study only.

โœฆ Synopsis

Determination of the structural characteristics of angiogenesis requires a procedure protective of the forming vascular fibers and the endothelial cell monolayer exhibiting cord formation. This report describes in situ fixation of angiogenic cultures in 96-well plates and the subsequent double embedding processing for electron microscopy. Cross sections of the monolayer are obtained without damage of the incipient capillaries.

๐Ÿ“œ SIMILAR VOLUMES

Preparation of cultured mammalian cells
Preparation of cultured mammalian cells for transmission and scanning electron microscopy using aclar film
โœ Kingsley, R. E. ;Cole, N. L. ๐Ÿ“‚ Article ๐Ÿ“… 1988 ๐Ÿ› Wiley (John Wiley & Sons) ๐ŸŒ English โš– 788 KB

Common methods for the preparation of cultured cells for concurrent light microscopy (LM), scanning electron microscopy (SEM), and transmission electron microscopy (TEM) are not completely satisfactory. This article describes how we grow mammalian cells on plastic disks made from Aclar film. Aclar i

A simple method for the removal of glass
A simple method for the removal of glass coverslips from flat-embedded cultured cells for transmission electron microscopy
โœ Howell, A. S. ;Sharma, S. ;Notter, M. F. D. ;Hansen, J. T. ๐Ÿ“‚ Article ๐Ÿ“… 1988 ๐Ÿ› Wiley (John Wiley & Sons) ๐ŸŒ English โš– 139 KB ๐Ÿ‘ 1 views

A variety of research applications require the use of cultured cells. However, it often is difficult to obtain well embedded monolayers of cultured cells for ultrastructural analysis, especially if sections parallel to the cell monolayer are desirable. Previously published techniques for obtaining p