Transmission electron microscopy of chromosomes by longitudinal section preparation: Application to fragile X chromosome analysis
✍ Scribed by Wen, Guang Y.; Jenkins, Edmund C.; Yao, Xiu-Lan; Yoon, David; Brown, W. Ted; Wisniewski, Henryk M.
- Publisher
- John Wiley and Sons
- Year
- 1997
- Tongue
- English
- Weight
- 321 KB
- Volume
- 68
- Category
- Article
- ISSN
- 0148-7299
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✦ Synopsis
We developed a method for the preparation of ultrathin longitudinal sections of chromosomes enabling TEM studies of whole chromosomes. By using a novel "repeat chill" method of exposing the glass slide and plastic block interface to liquid nitrogen, it was possible to separate consistently hardened epoxy resin-embedded chromosome spreads from glass slides for ultrathin longitudinal sectioning of entire spreads and of individual chromosomes. The method was applied to analyze the fragile X chromosome. The ultrastructure of centromeres, telomeres, fragile sites and other chromosomal areas can now be studied in detail.