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Transmembrane and cytoplasmic domain sequences demonstrate at least two expressed bovine MHC class I loci

✍ Scribed by Shirley A. Ellis; Karen A. Braem; W. Ivan Morrison

Book ID: 104670701
Publisher: Springer-Verlag
Year: 1992
Tongue: English
Weight: 729 KB
Volume: 37
Category: Article
ISSN: 0093-7711
DOI: 10.1007/bf00223544

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✦ Synopsis

We have used the polymerase chain reaction to amplify cDNA from expressed bovine major histocompatibility complex class I genes. Sequences obtained from transmembrane and cytoplasmic domains were used to identify the number of expressed alleles. Data from three animals suggest that there are four major expressed alleles, representing the products of two (or more) loci. We have also demonstrated the presence of an alternatively spliced mRNA, which has been observed in five animals. The alternative splicing removes exon 7 (the major site of class I phosphorylation), which predicts a truncated molecule with a cytoplasmic portion 16 amino acids shorter than usual. This phenomenon was detected for only a single class I allele within each individual.

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A cDNA library prepared from lymphocytes of a cow (E98), homozygous at major histocompatibility complex (MHC) loci (BoLA phenotype w10, KN104), was screened with a bovine MHC class I probe. Of the cDNA clones isolated, two, (2.1 and 5.1) were selected and showed divergence at both 5' and 3' termini.