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Translational Control in Production of Transposase and in Transposition of Insertion Sequence IS3

โœ Scribed by Yasuhiko Sekine; Naoki Eisaki; Eiichi Ohtsubo

Book ID
115626464
Publisher
Elsevier Science
Year
1994
Tongue
English
Weight
993 KB
Volume
235
Category
Article
ISSN
0022-2836

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Identification of the site of translatio
Identification of the site of translational frameshifting required for production of the transposase encoded by insertion sequence IS 1
โœ Sekine, Yasuhiko ;Nagasawa, Hiromichi ;Ohtsubo, Eiichi ๐Ÿ“‚ Article ๐Ÿ“… 1992 ๐Ÿ› Springer ๐ŸŒ English โš– 945 KB

Previous genetic analyses indicated that translational frameshifting in the--1 direction occurs within the run of six adenines in the sequence 5'-TTAAAAAACTC-3' at nucleotide positions 305-315 in IS 1, where the two out-of-phase reading frames insA and B'-insB overlap, to produce transposase with a

DNA sequences required for translational
DNA sequences required for translational frameshifting in production of the transposase encoded by IS 1
โœ Sekine, Yasuhiko ;Ohtsubo, Eiichi ๐Ÿ“‚ Article ๐Ÿ“… 1992 ๐Ÿ› Springer ๐ŸŒ English โš– 931 KB

The transposase encoded by insertion sequence IS1 is produced from two out-of-phase reading frames (insA and B'-insB) by translational frameshifting, which occurs within a run of six adenines in the -1 direction. To determine the sequence essential for frameshifting, substitution mutations were intr