β Scribed by T. A. King; A. Knox; J. D. G. McAdam
No coin nor oath required. For personal study only.
The translational and rotational dynamics of tobacco mosaic virus in sodium phosphate buffer (pH = 7.5) solutions has been investigated by polarized and depolarized light scattering Rayleigh linewidth studies. For concentrations ranging from 1.75 X 10-4 g ml-l to 0.25 X g ml-l the translational diffusion coefficient ( D T ) has been found to be slightly concentration dependent and extrapolation to zero concentration gives DO^^^^ = 0.34 f 0.01 X A full analysis of the polarized spectra obtained at high and low scattering angles and the depolarized spectra a t near zero scattering angle has enabled these techniques to be compared and the rotational diffusion constant DR to be determined. At a solution concentration of 1.75 X 10-4 g ml-1 a mean value is found to be DR~OOC = 350 f 30 s-l. These values of DT and DR are in approximate agreement with calculations based on models of the tobacco mosaic virus molecule as a cylindrical rod. cm*s-l.
π SIMILAR VOLUMES
## Abstract Two new techniques, amplitude modulation (AM) and frequency modulation (FM) of an electric field, are developed for the lightβscattering study of polymer solutions under ac electric fields. The AM technique makes it possible to observe accurately the frequency dependence of the intensit
## Abstract Dynamic light scattering experiments were performed on solutions of the bacteriophages T7 and T4B in order to obtain the rotational diffusion coefficients of these phages. Correlation functions were determined from the depolarized intensity scattered in the forward direction. The appara