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Translation of endogenous message in embryonic chick erythroid cell polysomes in a cell-free protein-synthesizing system

✍ Scribed by Nicholas Zagris

Publisher
Springer
Year
1977
Tongue
English
Weight
385 KB
Volume
15
Category
Article
ISSN
0006-2928

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✦ Synopsis

Polysomes from 5.5-day and 6.5-day embryonic chick erythroid cells contain messenger RNA (mRNA) which can be translated into products in a cell-free protein-synthesizing system. The products of the cell-free system coelectrophoresed with carrier globin chains from the cells from which the polysomal pellet was isolated. Wheat germ S30 fraction increases by 1.5--2.0 fold [3H] leucine incorporation into trichloroacetic acid-precipitable material directed by the endogenous mRNA on chick erythroid cell polysomes. The wheat germ probably provides a specific factor or factors absent in a shortage in the incubation medium.

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