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Transient state isoelectric focusing: Resolving power and zone resolution

โœ Scribed by Nicholas Catsimpoolas

Publisher
Elsevier Science
Year
1973
Tongue
English
Weight
361 KB
Volume
54
Category
Article
ISSN
0003-2697

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โœฆ Synopsis

A digital on-line data acquisition system has been used in conjunction with a scanning isoelectric focusing assembly for the direct calcuIation of the resolving power and zone resolution in transient state isoelectric focusing experiments. The application of the method to the evaluation of these two parameters in relation to (a) the applied electric field strength during focusing, and (b) to the diffusion in the absence of current is presented. A linear relationship between l/o' (where 0 is the standard deviation of the concentration distribution of a focused zone) and the applied voltage was demonstrated. The resolving power and resolution were generally improved with increased electric field strength.

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