Glucose and glutamine are the main nutrients used by mammalian cells i n culture. Each provides unique biosynthetic precursors but are complementary for production of other metabolites and energy. The transient and steady-state responses of hybridoma growth and metabolism to glucose pulse and step c
Transient responses of hybridoma cells to lactate and ammonia pulse and step changes in continuous culture
β Scribed by W. M. Miller; C. R. Wilke; H. W. Blanch
- Publisher
- Springer
- Year
- 1988
- Tongue
- English
- Weight
- 895 KB
- Volume
- 3
- Category
- Article
- ISSN
- 1615-7605
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Induction and repression kinetics of alphagalactosidase, galactose uptake system and Leloir pathway enzymes were studied in chemostat cultures by changing the medium feed from glucose (11 mM) to glucose and galactose (11 mM; 17 mM respectively) in the induction experiments; and from galactose (11 mM