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Transient gene expression in mammalian cells grown in serum-free suspension culture

✍ Scribed by Ernst-Jürgen Schlaeger; Klaus Christensen

Book ID
110257300
Publisher
Springer
Year
1999
Tongue
English
Weight
296 KB
Volume
30
Category
Article
ISSN
0920-9069

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## Abstract Transient expression of recombinant proteins in mammalian cell culture in a 100‐L scale requires a large quantity of plasmid that is very labour intensive to achieve with shake flask cultures and commercially available plasmid purification kits. In this paper we describe a process for p