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Transient gene expression in electroporatedPicea glaucaprotoplasts

✍ Scribed by F. Bekkaoui; M. Pilon; E. Laine; D. S. S. Raju; W. L. Crosby; D. I. Dunstan


Publisher
Springer
Year
1988
Tongue
English
Weight
543 KB
Volume
7
Category
Article
ISSN
0721-7714

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Factors affecting transient gene express
✍ Sarwan K. Dhir; Seema Dhir; Angus Hepburn; Jack M. Widholm πŸ“‚ Article πŸ“… 1991 πŸ› Springer 🌐 English βš– 932 KB

Electroporation was used to evaluate parameters affecting transient gene expression in Glycine max protoplasts. Protoplast viability and reporter enzyme activity for chloramphenicol acetyl transferase (CAT) and ß-glucuronidase (GUS) depended on the field strength employed. Maximum CAT and GUS activi