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Transient expression of the β-glucuronidase gene after biolistic transformation of the anaerobic fungus Neocallimastix frontalis

✍ Scribed by R. Durand; C. Rascle; M. Fischer; Michel Fèvre


Book ID
106006142
Publisher
Springer-Verlag
Year
1997
Tongue
English
Weight
485 KB
Volume
31
Category
Article
ISSN
0172-8083

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A microprojection protocol using the DuPont Biolistic(™) particle delivery system and the β-glucuronidase (GUS) reporter gene fused with the 35S promoter of Cauliflower mosaic virus (CaMV) was developed for Picea mariana callus. Comparison of four tungsten microprojectile sizes showed the highest tr