Transient expression of M-CSF is important for osteoclast-like cell differentiation in a monocytic leukemia cell line
✍ Scribed by Hiromoto Aoki; Hidehiko Akiyama; Hiromi Hosoya; Masahiko Souda; Toshie Morioku; Tohru Marunouchi
- Publisher
- John Wiley and Sons
- Year
- 1997
- Tongue
- English
- Weight
- 282 KB
- Volume
- 64
- Category
- Article
- ISSN
- 0730-2312
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✦ Synopsis
Cells of U937, a human monocytic leukemia cell line, differentiate into macrophages by treatment with 12-o-tetradecanoylphorbol-13-acetate (TPA), whereas cells treated with 1a,25-dihydroxyvitamin D3 [1,25-(OH) 2 D 3 ] continue to grow without undergoing differentiation. When U937 cells were successively treated with TPA and 1,25-(OH) 2 D 3 , tartrate-resistant acid phosphatase-positive multinucleated cells appeared at 5 days after the treatment. These osteoclast-like cells released a soluble form of 45 Ca from 45 Ca-labeled bone particles. These cells were not formed when the order of treatment with TPA and 1,25-(OH) 2 D 3 was reversed. Use of either dexamethasone or interferon-g (IFN-g) was effective in inhibiting the formation of these osteoclast-like cells. The expression of c-src, c-fms, and macrophage colony stimulating factor (M-CSF) was induced by TPA treatment; however, TPA-induced M-CSF gene transcription was attenuated by the subsequent addition of 1,25-(OH) 2 D 3 . Furthermore, both dexamethasone and IFN-g impaired the attenuation of M-CSF expression, suggesting that the transient expression of M-CSF may be important for the formation of osteoclast-like cells.