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Transient expression of a chloramphenicol acetyltransferase gene following transfection ofPhysarum polycephalummyxamoebae

โœ Scribed by Karen McCurrach; L. Anne Glover; Norman Hardman

Book ID
104741978
Publisher
Springer-Verlag
Year
1988
Tongue
English
Weight
649 KB
Volume
13
Category
Article
ISSN
0172-8083

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โœฆ Synopsis

A plasmid was constructed containing a replication origin sequence from the Physarum ribosomal DNA molecule, and a bacterial chloramphenicol acetyltransferase (CAT) gene linked to a putative promoter of the long terminal repeat (LTR) of the Physarum "HpalI-repeat" element. The plasmid was transfected into Physarum myxamoebae either by electroporation or CaC12 treatment. In both cases significant transient levels of CAT gene expression were detected. Results were compared with those obtained with plasmids in which CAT gene expression was driven by eukaryotic virus promoters.

๐Ÿ“œ SIMILAR VOLUMES

Transient expression of chloramphenicol
Transient expression of chloramphenicol acetyltransferase (CAT) gene in barley cell cultures and immature embryos through microprojectile bombardment
โœ K. K. Kartha; R. N. Chibbar; F. Georges; N. Leung; K. Caswell; E. Kendall; J. Qu ๐Ÿ“‚ Article ๐Ÿ“… 1989 ๐Ÿ› Springer ๐ŸŒ English โš– 864 KB

Transient expression of chloramphenicol acetyl transferase gene has been detected in cultured barley (Hordeum vulgare L. cv. Heartland) cells and freshly isolated immature zygotic embryos (cv. Ellice) following the introduction of the gene by microprojectile bombardment. The DNA expression vector us