Transient effects in the nanosecond laser photolysis of carboxyhemoglobin: “CAGE” recombination annd spectral evolution of the protein

✍ Scribed by B. Alpert; S. El Mohsni; L. Lindqvist; F. Tfibel

• Publisher: Elsevier Science
• Year: 1979
• Tongue: English
• Weight: 544 KB
• Volume: 64
• Category: Article
• ISSN: 0009-2614
• DOI: 10.1016/0009-2614(79)87265-6

✦ Synopsis

France Reccibed 7 &lay 1979 The transient light absorption changes at 400400 nm observed foIlowin~ photodlssociation of hum;ln adulr cabox)'hemogtobin in aqueous solution (pH 7.0) by pulsed hser irradiation (7 ns fwhm. h = 529 nm) reveal that geminate recombination is occurring durin g -100 ns after the dissociation. They also show that the spectrum of the pbotodeIigsted hemoglobin initially is sIig,htly different from that of stable deoxyhemoglobin but de;eiops into the Iatter in =5 ps. The recombination kinetics v3ry with the ewitation conditions (duration, intensity).