TRANSIENT APOPTOSIS ELICITED BY INSULIN IN SERUM-STARVED GLIOMA CELLS INVOLVES Fas/Fas-L AND Bcl-2
✍ Scribed by Bei-Chang Yang; Yuh-Sheng Wang; Cheng-Hsin Wang; Hsi-Hui Lin; Ming-Jer Tang; Tsae-Lian Yang
- Book ID
- 102567599
- Publisher
- Elsevier Science
- Year
- 1999
- Tongue
- English
- Weight
- 392 KB
- Volume
- 23
- Category
- Article
- ISSN
- 1065-6995
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✦ Synopsis
The expression of fas gene in glioma cells varies with growth stage. When insulin-elicited transient apoptosis of glioma cells was in progress, the expression of fas gene increased at both transcriptional and translational levels. In contrast, the expression of fas-L gene in glioma cells remained constant. Apoptosis occurred in the cells having high level of surface Fas protein.
When the expression of Fas-L in U-373MG cells was suppressed by ribozyme, the insulinelicited transient apoptosis vanished. Overexpression of Bcl-2 in U-373MG cells did not alter significantly the cell cycle progression and the expression of fas gene. However, these cells were resistant to insulin-trigged death. Therefore, insulin-elicited apoptosis involved Fas-related death signal, and which could be prevented by the protective effect of Bcl-2.