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Transglutaminase-like activity in Chrysanthemum leaf explants cultivatedin vitroin relation to cell growth and hormone treatment

✍ Scribed by Marcel Aribaud; Monique Carré; Josette Martin-Tanguy


Book ID
104618729
Publisher
Springer Netherlands
Year
1995
Tongue
English
Weight
564 KB
Volume
16
Category
Article
ISSN
0167-6903

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✦ Synopsis


In Chrysanthemum leaf explants cultivated in vitro the capacity to covalently link polyamines to protein substances exists. This plant enzyme activity shows some similarities with mammalian transglutaminases. In foliar explants cultured on a medium promoting bud or root formation increasing levels of transglutaminase-like activity occurred during the first days of culture when cell multiplication was rapid then the levels declined as the rate of cell division decreased and differentiation occurred. Undifferentiated callus exhibited low transglutaminase-like activity. Transglutaminase-like activity also increased in rapidly proliferating and growing organs (roots and buds initiated from the foliar explants) and decreased during maturity. The relationship among transglutaminases-like activity, cell division, bud and root formation is discussed.