𝔖 Bobbio Scriptorium
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Transgenic poplars: expression of chimeric genes using four different constructs

✍ Scribed by Jean Charles Leple; Ana Cristina Miranda Brasileiro; Marie France Michel; Francis Delmotte; Lise Jouanin

Book ID
104674886
Publisher
Springer
Year
1992
Tongue
English
Weight
869 KB
Volume
11
Category
Article
ISSN
0721-7714

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✦ Synopsis

Leaf or stem explants of a hybrid poplar clone (Populus tremula X Populus alba), sensitive to Agrobacterium tumefaciens, were co-cultivated either by an octopine or a nopaline disarmed A. tumefaciens modified strain. Transformed poplar shoots were readily regenerated from explants. The protocol was improved using the nopaline disarmed strain C58/pMP90 with the binary vector pBI121. This protocol was then used to test three other vectors. The first one, possessing a nptII gene fused to the CaMV 19S promoter, permitted regeneration of transformed shoots in presence of 50 to 100 mg/l kanamycin. The two other vectors carried an additional nptII gene under the control of the CaMV 35S or CaMV 35S promoter with a double enhancer sequence (CaMV 70). CaMV 70 promoter provided consistently higher level of gene expression than the other promoters in both callus and leaf tissues.

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## Abstract Identification of transgenics still requires PCR and genomic Southern blot hybridization of genomic DNA isolated from tail pieces. Furthermore, identification of transgene‐expressing transgenics (hereafter called β€œexpressor”) requires mRNA analyses (RT‐PCR and Northern blot hybridizatio