Transgenic carnation plants obtained byAgrobacterium tumefaciens-mediated transformation of petal explants

✍ Scribed by Anne-Claire Altvorst; Herma Koehorst; Jan Jong; Hans J. M. Dons

• Publisher: Springer
• Year: 1996
• Tongue: English
• Weight: 938 KB
• Volume: 45
• Category: Article
• ISSN: 0167-6857
• DOI: 10.1007/bf00048762

✦ Synopsis

Transgenic carnation plants were obtained after infection of petal explants with the supervirulent Agrobacterium tumefaciens strain AGLO. Southern blot techniques confirmed the transgenic nature of four transformed plants. The expression of the gus gene was verified in these plants by histochemical assays on selected shoots. It was very difficult to transfer the transgenic plants to the greenhouse due to vitrification and premature flowering.