Introduction To Transgenesis -- Transgenic Rodents And The Study Of The Central Nervous System -- Transgenic Animals And The Study Of Cancer -- Transgenic Animals And The Study Of The Immune System -- Commercial And Agricultural Applications Of Animal Transgenesis -- P Element-mediated Germ-line Transformation Of Drosophila -- Gene Transfer By Microinjection In The Zebra Fish Brachydanio Rerio -- Transgenic Induction In Salmonid And Tilapia Fish -- Overview Of Transgenic Mouse Production -- Transgene Design -- Isolation Of Dna Fragments For Microinjection -- Establishing A Colony For Efficient Production Of Transgenic Mice -- Mating Mice -- Anesthetizing Mice -- Vasectomizing A Mouse -- Preparation Of Culture Media For Fertilized One-cell Mouse Eggs -- Collection Of Fertilized One-cell Mouse Eggs For Microinjection -- Microinjection Of Cloned Dna Fragments Into Fertilized One-cell Mouse Eggs. 1, Manual Injection --^ Microinjection Of Cloned Dna Fragments Into Fertilized One-cell Mouse Eggs. 2, Automatic Injection -- Delivery Of Microinjected Eggs To Surrogate Mothers By Oviduct Transfer -- Caesarean Section And Fostering -- In Vitro Fertilization Of Mouse Eggs -- In Vitro Isolation Of Murine Embryonic Stem Cells -- Production Of Chimeras Derived From Murine Embryonic Stem Cells -- Cryopreservation Of Transgenic Mouse Lines -- Introduction To The Physiology And Husbandry Of The Rat -- Mating Of Rats -- Vasectomy Of The Male Rat -- Harvest Of Fertilized One-cell Rat Eggs -- Implantation Of Microinjected Eggs -- Cryopreservation Of Transgenic Rat Lines -- Production Of Transgenic Sheep -- Cryopreservation Of Transgenic Sheep Lines -- Isolation Of Genomic Dna From Tail Tissue -- Slot Blotting Of Genomic Tail Dna -- Genomic Analysis Of Transgenic Animals By The Polymerase Chain Reaction -- Genomic Analysis Of Transgenic Animals : Southern Blotting --^ The Preparation Of Rna From The Tissues Of Transgenic Animals -- Northern Blotting -- Rna Slot Blotting -- Analysis Of Gene Expression By Pcr -- Nuclear Run-on Analysis Of Transcription -- S1 Nuclease Protection Mapping -- Primer Extension -- In Situ Hybridization Analysis Of Transgenic Tissues -- Immunohistochemical Analysis Of Transgene Expression -- Postmortem Examination Of Transgenic Mice -- Histological Examination Of Transgenic Mice -- Reporter Enzyme Assays -- Restriction Endonuclease Digestion Of Dna -- Agarose Gel Electrophoresis -- Working With Radioactivity In The Transgenic Animal Laboratory -- Random Primed Labeling Of Dna -- Filter Hybridization -- Dna Transfection. Edited By David Murphy, David A. Carter. Includes Bibliographical References And Index.