Transforming growth factor β1 stimulates inorganic pyrophosphate elaboration by porcine cartilage

✍ Scribed by Ann K. Rosenthal; Herman S. Cheung; Lawrence M. Ryan

• Publisher: John Wiley and Sons
• Year: 1991
• Tongue: English
• Weight: 774 KB
• Volume: 34
• Category: Article
• ISSN: 0004-3591
• DOI: 10.1002/art.1780340717

✦ Synopsis

The overproduction of inorganic pyrophosphate (PP,) by cartilage is thought to be a key element in the formation of calcium pyrophosphate dihydrate (CPPD) crystals in joints, and the subsequent development of pseudogout or chondrocalcinosis. We report herein that transforming growth factor Pl (TGFPl), alone and in synergy with epidermal growth factor (EGF) or TGFa, markedly stimulates PP, elaboration by porcine articular cartilage in organ culture and monolayer culture. This effect is not seen with platelet-derived growth factor, basic fibroblast growth factor, or insulin-like growth factor types 1 and 2, substances which also affect chondrocyte metabolism or are mitogenic. TGFPl produces only a modest increase in nucleoside triphosphate pyrophosphohydrolase (NTPPPH), a chondrocyte ectoenzyme that produces PP,; this implies the existence of other pathways for PP, elaboration. TGFPl is presedt in joint fluid and cartilage. TGFPl, TGFa, and EGF are the first known physiologic modifiers of cartilage PP, production. They provide a novel model for the study of CPPD crystal formation in cartilage, as well as new From the Division of Rheumatology, Department of Medicine,