Transforming Growth Factor-β1 Has both Promoting and Inhibiting Effects on Induction of DNA Synthesis in Human Fibroblasts
✍ Scribed by Junya Fukami; Kazue Tsuji; Akemi Ueno; Toshinori Ide
- Book ID
- 115601923
- Publisher
- Elsevier Science
- Year
- 1995
- Tongue
- English
- Weight
- 395 KB
- Volume
- 216
- Category
- Article
- ISSN
- 0014-4827
No coin nor oath required. For personal study only.
📜 SIMILAR VOLUMES
We investigated the influence of transforming growth factor-@ (TCF-0) on DNA synthesis in human fetal fibroblasts, as measured by the incorporation of [3H] thymidine and cell replication. In serum-free medium, without additional peptide growth factors, TGF-@ had no action on thymidine incorporation.
## Abstract Platelet‐derived growth factor (PDGF) and transforming growth factor β (TGF‐β), potent modulators of mesenchymal cell growth and differentiation, are often colocalizable in vivo. Previous in vitro studies in fibroblastic cell lines have shown variable, even antagonistic effects of TGF‐β
A single-stranded 27-mer phosphorothioate oligodeoxynucleotide (ssPT) containing the transforming growth factor- (TGF-) response element was synthesized. Rat fetal lung fibroblasts were stably transfected with the ColCat 3.6 plasmid, which contains a portion of the 5Ј-flanking region of the pro␣1(