Transforming activities of sodium fluoride in cultured Syrian hamster embryo and BALB/3T3 cells

✍ Scribed by Claude Lasne; Yao -Ping Lu; Ivan Chouroulinkov

• Publisher: Springer
• Year: 1988
• Tongue: English
• Weight: 809 KB
• Volume: 4
• Category: Article
• ISSN: 0742-2091
• DOI: 10.1007/bf00058739

✦ Synopsis

The transforming activity of sodium fluoride was studied in the SHE and the BALB/3T3 cell culture systems. Initiating and promoting activities were then investigated by means of the orthogonal methodology. Sodium fluoride was found to induce morphological transformation of SHE cells seeded on a feeder layer of X-irradiated cells at high concentrations (75-125 #g/ml). When the cells were seeded in the absence of a feeder-layer, the transformation frequencies increased in a dose-dependent manner with the concentrations of sodium fluoride ranging from 0 to the highly toxic concentration of 200 I~g/ml. In the BALB/3T3 cell system, sodium fluoride was negative in the standard Kakunaga procedure, while through the experiment designed by table L8 (27) of the orthogonal method, an initiating-like effect and a weak promoting activity were detected within the concentrations ranging from a 25 I.tg/ml to a 50 #g/ml concentration which is highly toxic for BALB/3T3 cells. From these results, it is suggested that, besides a genetic mode of action, sodium fluoride could possibly act through a non-genotoxic mechanism.