Transformation ofUstilago maydisby a plasmid containing yeast 2-micron DNA
โ Scribed by Goeffrey R. Banks
- Book ID
- 104741916
- Publisher
- Springer-Verlag
- Year
- 1983
- Tongue
- English
- Weight
- 418 KB
- Volume
- 7
- Category
- Article
- ISSN
- 0172-8083
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
Hybrid plasmids have been constructed containing segments of the yeast plasmid 2 ฮผ DNA, the yeast ochre-suppressing SUP4.0 gene and the bacterial plasmid pBR322. Yeast transformation is detected with a host containing multiple ochre auxotrophic mutations. The transformed SUP4.0 gene is active and ca
The 2 micron circular DNA from S. cerevisiae has been cloned on bacteriophage lambda. The two forms of circular DNA which exist in equilibrium due to recombination between inverted repeat sequences were separated as stable clones, and a map of targets for restriction endonucleases EcoRI, HindIII and
Partially purified cell-free extracts prepared from growing cells of the yeast Saccharomyces cerevisiae catalyze DNA synthesis directed by the chimeric plasmid BTYP-2 containing the whole 2 ฮผm DNA sequence. The biochemical properties of the reaction have been examined and inactivation studies indica
Yeast DNA pools were prepared by ligating partial Sau3A genomic digests from strains carrying various MAL genes into the BamHI site of the yeast-Escherichia coli shuttle vector YRp7. They were used to transform recipient yeast strains that could not utilize maltose since they lacked a classical MAL