Transformation ofMedicago arboreaL. with anAgrobacterium rhizogenesbinary vector carrying the hygromycin resistance gene

✍ Scribed by Francesco Damiani; Sergio Arcioni

• Book ID: 104658397
• Publisher: Springer
• Year: 1991
• Tongue: English
• Weight: 874 KB
• Volume: 10
• Category: Article
• ISSN: 0721-7714
• DOI: 10.1007/bf00193146

✦ Synopsis

Plants of Medicago arbores have been infected with

A~bacterium rbizogenes strain LBA9402 hazbouring the plasmids Ri 1855 and AGS125 carrying a gene conferring resistance to the antibiotic hygromycin. About 70~ of the hairy roots showed callus formation on hygromyein-sopplemented medium. Regeneration took place on antibiotic free medium only. Plantlels suitable for transfer to soil were obtained after the manual removal of most of the leaves. Plant morphology showed the usual alterations induced by the Ri plasmld; moreover, two years after soil-transfer, transformants have not flowered. Molecular analysis indicates the presence of T-DNA from both pAGSI25 and p1855. The expression of the hygromycin phosphotransfersse gene allowed callus and protoplasts of t.ransformed plants to grow on media supplemented with the antibiotic. This trait will be utilized as a marker in protoplast fusion between M.edicsgo arbores and Medicsgo sstiva (alfalfa).