Transformation of the yeastKluyveromyces lactisby new vectors derived from the 1.6μm circular plasmid pKD1

✍ Scribed by Michele Maria Bianchi; Claudio Falcone; Chen Xin Re; Micheline Wéslowski-Louvel; Laura Frontali; Hiroshi Fukuhara

• Book ID: 104772931
• Publisher: Springer-Verlag
• Year: 1987
• Tongue: English
• Weight: 900 KB
• Volume: 12
• Category: Article
• ISSN: 0172-8083
• DOI: 10.1007/bf00436877

✦ Synopsis

The circular plasmid pKD1 (or 1.6 gm DNA) has recently been isolated from Kluyveromyces drosophilarum. This plasmid appears to have a functional organization analogous to that of the 2 g DNA of Saccharomyces cerevisiae, although the respective nucleotide sequences show little homology, pKD1 can be transferred toKluyverornyces lactis where it is replicated stably. Using recombinant molecules derived from pKD1, a practical transformation system has been developed for Kluyveromyces lactis, with an efficiency and stability comparable to the 2#-based Saccharomyces cerevisiae transformation system.