Transformation of single myeloid precursor cells by the malignant histiocytosis sarcoma virus (MHSV): Generation of growth-factor-independent myeloid colonies and permanent cell lines
✍ Scribed by K. Klingler; G. R. Johnson; N. A. Nicola; G. Arman; N. Kluge; W. Ostertag
- Publisher
- John Wiley and Sons
- Year
- 1988
- Tongue
- English
- Weight
- 700 KB
- Volume
- 135
- Category
- Article
- ISSN
- 0021-9541
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✦ Synopsis
Direct single-cell assays for oncogenic transformation are available for fibroblasts but not for other cell types. Using malignant histiocytosis sarcoma virus (MHSV), a member of t h e ras family of retroviruses, in vivo-infected granulocytehacrophage and macrophage precursor cells lost the requirement for externally added hematopoietic growth factors. Factor-independent growth was demonstrated by colony-transfer experiments. More thaq 25% of the independent colonies were established as permanent macrophage cell lines following a phase of adaptation to tissue culture conditions. Factor-independent colony growth was also obtained by in vitro infection of single cells. As many as 50% of all myeloid precursor cells were target cells for MHSV as measured by this assay. About 2 x of these colony-forming cells acquired growth factor independence and immortality after in vitro infection. Cell lines derived from these colonies did not require adaptation to tissue culture conditions.