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Transformation of rabbit kidney cells by bkv(mm) human papovavirus

✍ Scribed by David H. Mason JR.; Kenneth K. Takemoto


Publisher
John Wiley and Sons
Year
1977
Tongue
French
Weight
528 KB
Volume
19
Category
Article
ISSN
0020-7136

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✦ Synopsis


Abstract

Primary rabbit kidney cells were transformed by BKV(MM), a papovavirus isolated from the urine of a child with the Wiskott‐Aldrich syndrome. The transformed cells contained BK T‐antigen, but no antigen that reacted with SV40 U‐antiserum. The transformed cells failed to produce tumors in nude mice, and BKV (MM) was not rescued from transformed cells by cell fusion or chemical induction methods. The transformed cells supported the growth of rabbit kidney vacuolating virus (RKV), and could be used to quantitate RKV by plaque formation under an agar overlay.