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Transformation of mouse cells after infection with ultraviolet irradiation-inactivated herpes simplex virus type 2

✍ Scribed by Ann Lewis Boyd; Thomas W. Orme

Publisher
John Wiley and Sons
Year
1975
Tongue
French
Weight
896 KB
Volume
16
Category
Article
ISSN
0020-7136

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✦ Synopsis

Abstract

A transformed mouse cell line (H238) was obtained following the infection of 238 mouse cells with ultraviolet (UV) irradiation‐inactivated herpes simplex virus type 2 (HSV‐2). The transformed cells produced tumors with a 100% incidence within 8 weeks in 6‐week‐old syngeneic BALB/c mice at an inoculum of 1 × 10^6^. Indirect immunofluorescence (IF) tests revealed the presence of HSV antigens in the transformed cells. Antibodies to HSV‐2 were found in the sera of tumor‐bearing animals by neutralization and IF techniques. Neither HSV‐2 infectious virus nor viral antigens could be detected by the transfer of transformed‐cell DNA into permissive cells.

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