A simple method that allows the rapid preparation of oligo dG-tailed plasmid vectors is presented. The procedure involves purification of the tailed molecules by hybridization to oligo dC-cellulose followed by a stepwise thermal elution. The resulting plasmid is virtually devoid of transformation ac
β¦ LIBER β¦
Transformation of a cloning vector, pUB110, into Bacillus anthracis
β Scribed by Sou-ichi Makino; Chihiro Sasakawa; Ikuo Uchida; Nobuyuki Terakado; Masanosuke Yoshikawa
- Book ID
- 109313878
- Publisher
- John Wiley and Sons
- Year
- 1987
- Tongue
- English
- Weight
- 481 KB
- Volume
- 44
- Category
- Article
- ISSN
- 0378-1097
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