Isolation and transformation of uracil a
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Lakshmi Akileswaran; Margaret Alic; Edith K. Clark; Jason L. Hornick; Michael H.
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Article
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1993
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Springer-Verlag
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English
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Uracil auxotrophs of Phanerochaete chrysosporium were isolated using 5-fluoroorotate resistance as a selection scheme. The ura3 auxotrophs deficient in orotidylate decarboxylase and ura5 auxotrophs deficient in orotate phosphoribosyl transferase were characterized by enzyme assays and complementatio