Transformation as a tool for studying the epidemiology oftetdeterminants inStreptococcus pneumoniae.

✍ Scribed by G. Pozzi; M. Stellini; L. Marri; A.M. Molina

• Publisher: Springer
• Year: 1986
• Tongue: English
• Weight: 566 KB
• Volume: 2
• Category: Article
• ISSN: 0393-2990
• DOI: 10.1007/bf00157016

✦ Synopsis

Streptococcus pneumoniae

Tetracycline 'Transformation of pneumococcus was used to detect homology among tetracycline resistance determinants of clinical isolates ,of Streptococcus pneumoniae. A strain of pneumococcus containing a mutated tet determinant (tet-3), of class M, integrated into the chromosome was used as a recipient in transformation experiments, where d,on,or DNA was from the tetracycline resistant isolates. 34/34 strains appeared to have tet ,determinants homologous to tet-3 (i.e. tet M).

Still using transformation i,t was possible to determine that the tet-3 transforming activity of DNA from Tng16 and S. pneumo,niae BM6001 was contained in a 5 kb HincII fragment. For this purpose a transformation technique where donor DNA was directly taken from low melting point agarose gels was standardized and used.