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Transformation and expression of a cloned δ-endotoxin gene in bacillus thuringiensis

✍ Scribed by Lereclus, D. (author);Arantès, O. (author);Chaufaux, J. (author);Lecadet, M. M. (author)

Book ID
109314912
Publisher
John Wiley and Sons
Year
1989
Tongue
English
Weight
468 KB
Volume
60
Category
Article
ISSN
0378-1097

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✍ Schurter, Walter ;Geiser, Martin ;Mathé, Danièle 📂 Article 📅 1989 🏛 Springer 🌐 English ⚖ 565 KB

Electroporation was used as a method to transform intact cells of Bacillus thuringiensis and B. cereus. With our optimized method a range of plasmid vectors could be transformed into strains of B. thuringiensis at frequencies of up to 10(7) transformants/micrograms DNA. This high frequency allows cl