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Transferring genes into cultured mammalian embryos by electroporation

✍ Scribed by Masanori Takahashi; Tadashi Nomura; Noriko Osumi


Book ID
108944126
Publisher
John Wiley and Sons
Year
2008
Tongue
English
Weight
527 KB
Volume
50
Category
Article
ISSN
0012-1592

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Electroporation, the reversible breakdown of cell membranes caused by a high-voltage discharge, is a rapid, simple, and efficient method for introducing DNA into mammalian cells. An instrument for electroporation which permits the high-voltage discharge waveform to be varied with respect to rise tim