Transferase Activity of a β-Glycosidase from Thermus thermophilus: Specificities and Limits – Application to the Synthesis of β-[1→3]-Disaccharides

✍ Scribed by Violaine Chiffoleau-Giraud; Petra Spangenberg; Michel Dion; Claude Rabiller

• Publisher: John Wiley and Sons
• Year: 1999
• Tongue: English
• Weight: 315 KB
• Volume: 1999
• Category: Article
• ISSN: 1434-193X
• DOI: 10.1002/(sici)1099-0690(199904)1999:4<757::aid-ejoc757>3.0.co;2-3

✦ Synopsis

The aim of this paper is to test the ability of a β-glycosidase regioselectivity of the reactions is mainly of the β-[1Ǟ3] type, good yields are obtained for the synthesis of 2-nitrophenyl-from Thermus thermophilus to catalyse transglycosylation reactions in the presence of nitrophenyl glycosides as donors β-D-galactopyranosyl-[1Ǟ3]-β-D-galactopyranoside and 2nitrophenyl-β-D-glucopyranosyl-[1Ǟ3]-β-D-glucopyranoside. and other monosaccharides as acceptors. Our results show that this enzyme is able to induce such reactions either with Conversely, in the presence of p-nitrophenylfucoside, the autocondensation is very limited, and with methyl-α-D-nitrophenylgalactosides, -glucosides and -fucosides. With the two former donors, the autocondensation of the donor, which galactoside as an acceptor, the regioselectivity is mainly of the β-[1Ǟ6]-type resulting in the synthesis of methyl-β-D-thus acts also as an acceptor, is faster than the transglycosylation with other acceptors. Furthermore, as the fucopyranosyl-[1Ǟ6]-α-D-galactopyranoside.