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Transfer of the drug-resistance transposon Tn5 to Rhizobium

✍ Scribed by BERINGER, J. E.; BEYNON, J. L.; BUCHANAN-WOLLASTON, A. V.; JOHNSTON, A. W. B.


Book ID
109711529
Publisher
Nature Publishing Group
Year
1978
Tongue
English
Weight
260 KB
Volume
276
Category
Article
ISSN
0028-0836

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Mutagenesis ofAlcaligenes eutrophusby in
✍ Sheela Srivastava; Marlies Urban; BΓ€rbel Friedrich πŸ“‚ Article πŸ“… 1982 πŸ› Springer 🌐 English βš– 513 KB

Drug-resistance element Tn5 coding for kanamycin resistance was used for mutagenesis of Alcaligenes eutrophus strain H16. The vehicle for introducing Tn5 into A. eutrophus was plasmid pJB4JI harboured by Escherichia coli. Kanamycin-resistant transconjugants occurred at a frequency of approximately 5