✦ LIBER ✦
Transfer of nitrogen fixation genes intoPseudomonas putidaisolated from Finnish tundra soil
✍ Scribed by Hannu Lehtinen; Pekka Mäntsälä
- Book ID
- 104770619
- Publisher
- Springer Netherlands
- Year
- 1981
- Tongue
- English
- Weight
- 443 KB
- Volume
- 47
- Category
- Article
- ISSN
- 0003-6072
No coin nor oath required. For personal study only.
✦ Synopsis
The plasmid pRD 1 containing the n/f genes from Klebsiella pneumoniae was transferred by conjugation from Escherichia coli to Pseudomonasputida isolated from the tundra soil. 6-Cyanopurine, acetylene reduction and immunological tests showed that the n/f genes were not expressed in P. putida. Existence of the n/f genes in P. putida transconjugants was detected by transferring them to E. coli C600, which does not fix nitrogen. Existence of the n/f genes in E. coli C600 transconjugants was detected immunologically and by acetylene reduction tests.