Isolation of transfer-negative nif-plasm
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Göttfert, M. ;Klingmüller, W.
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Article
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1985
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Springer
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English
⚖ 536 KB
Strain JC5466 of Escherichia coli K12 harbouring the nitrogen fixation plasmid pCE1 was lysogenized with bacteriophage Mu cts, followed by partial induction and infection with bacteriophage PRD1. This made it possible to obtain transfer-defective derivatives of pCE1, carrying Mu prophage. These deri