✦ LIBER ✦

Transfer of eukaryotic expression plasmids to mammalian host cells by bacterial carriers

✍ Scribed by Siegfried Weiss; Trinad Chakraborty

Publisher: Elsevier Science
Year: 2001
Tongue: English
Weight: 74 KB
Volume: 12
Category: Article
ISSN: 0958-1669
DOI: 10.1016/s0958-1669(00)00247-0

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✦ Synopsis

The concept of transkingom transfer of DNA from bacteria to other organisms has recently been extended to include eukaryotic host cells. Intracellular bacteria have been shown to transfer eukaryotic expression plasmids to mammalian host cells in vitro and in vivo. This can be used to induce immune responses towards protein antigens encoded by the plasmid, to complement genetic defects or even to direct the production of proteins in appropriate organs. The ease of generating such vehicles makes this a highly attractive area for further research.

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## Abstract ## Background Cystic fibrosis (CF) is one of the most common monogenic disorders in the caucasian population. Gene therapy for CF is principally feasible and bacterial transfer systems might provide novel possibilities for therapy. However, transfection efficiencies are low and need to