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Transfection of partially purified plasmid DNA for high level transient protein expression in HEK293-EBNA cells

✍ Scribed by J.L. Wright; M. Jordan; F.M. Wurm


Book ID
119524388
Publisher
Elsevier Science
Year
2003
Tongue
English
Weight
460 KB
Volume
102
Category
Article
ISSN
0168-1656

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We describe in detail a robust, sensitive, and versatile functional assay for G-protein-coupled receptors (GPCRs) expressed in human embryonic kidney (HEK) 293-EBNA (Epstein-Barr virus nuclear antigen) (designated 293E) cells. The ability to grow these cells in suspension, in conjunction with the us