Transfection of normal human skin fibroblasts with human α1(I) and α2(I) collagen gene constructs and evidence for their coordinate expression

✍ Scribed by Ryu-Ichiro Hata

• Publisher: Elsevier Science
• Year: 1995
• Tongue: English
• Weight: 324 KB
• Volume: 19
• Category: Article
• ISSN: 1065-6995
• DOI: 10.1006/cbir.1995.1124

✦ Synopsis

Abstract

Type I collagen is composed of two α1(I) chains and one α2(I) chain that together form a unique triple helical structure. The genes for these chains are located on different chromosomes but their expression is tightly regulated. In order to investigate the mechanism of regulation of coordinate expression of these genes, I examined conditions for the efficient transfection of normal human skin fibroblasts with luciferase reporter gene constructs containing noncoding region of the first exon and the upper 500 base pairs sequence of the α1(I) or α2(I) gene. Expression ratio of these two reporter gene constructs was two to one, indicating these regions of α1(I) and α2(I) genes contain essential regulatory elements for the coordinate expression of α1(I) and α2(I) genes located on different chromosomes.